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김민지님의 글입니다.
>안녕하세요 <div> </div><div>물리화학적 성질 분석 및 성분 분석 가능 여부 문의드립니다. 물리화학적 성분 아래 내용에서 어떤 것들이 평가 가능할까요? </div><div>돼지태반 추출물은 분말 상태로 되어있습니다.</div><div> </div><div><div class="x_elementToProof" data-olk-copy-source="MessageBody" style="border: 0px; font-variant-numeric: inherit; font-variant-east-asian: inherit; font-variant-alternates: inherit; font-variant-position: inherit; font-variant-emoji: inherit; font-stretch: inherit; font-size: 12pt; line-height: inherit; font-family: Aptos, Aptos_EmbeddedFont, Aptos_MSFontService, Calibri, Helvetica, sans-serif; font-optical-sizing: inherit; font-size-adjust: inherit; font-kerning: inherit; font-feature-settings: inherit; font-variation-settings: inherit; margin: 0px; padding: 0px; vertical-align: baseline;">식약처 화장품 위해평가 보고서 작성을 위해 물리 화학적 성질 데이터가 필요합니다.</div><div class="x_elementToProof" style="border: 0px; font-variant-numeric: inherit; font-variant-east-asian: inherit; font-variant-alternates: inherit; font-variant-position: inherit; font-variant-emoji: inherit; font-stretch: inherit; font-size: 12pt; line-height: inherit; font-family: Aptos, Aptos_EmbeddedFont, Aptos_MSFontService, Calibri, Helvetica, sans-serif; font-optical-sizing: inherit; font-size-adjust: inherit; font-kerning: inherit; font-feature-settings: inherit; font-variation-settings: inherit; margin: 1em 0px; padding: 0px; vertical-align: baseline;">[물질명, IUPAC명, CAS No., 화학식, 분자량, 순도(분석방법), 구조식, 물리적 성상, 녹는점, 끓는점, 밀도, 용해도, Log Pow, 헨리상수, 동의어]</div></div><div class="x_elementToProof" style="border: 0px; font-variant-numeric: inherit; font-variant-east-asian: inherit; font-variant-alternates: inherit; font-variant-position: inherit; font-variant-emoji: inherit; font-stretch: inherit; font-size: 12pt; line-height: inherit; font-family: Aptos, Aptos_EmbeddedFont, Aptos_MSFontService, Calibri, Helvetica, sans-serif; font-optical-sizing: inherit; font-size-adjust: inherit; font-kerning: inherit; font-feature-settings: inherit; font-variation-settings: inherit; margin: 1em 0px; padding: 0px; vertical-align: baseline;">성분분석 의뢰 내용은 다음과 같습니다.</div><div class="x_elementToProof" style="border: 0px; font-variant-numeric: inherit; font-variant-east-asian: inherit; font-variant-alternates: inherit; font-variant-position: inherit; font-variant-emoji: inherit; font-stretch: inherit; font-size: 12pt; line-height: inherit; font-family: Aptos, Aptos_EmbeddedFont, Aptos_MSFontService, Calibri, Helvetica, sans-serif; font-optical-sizing: inherit; font-size-adjust: inherit; font-kerning: inherit; font-feature-settings: inherit; font-variation-settings: inherit; margin: 1em 0px; padding: 0px; vertical-align: baseline;">전처리도 해주시는지 궁금합니다.</div><div class="x_elementToProof" style="border: 0px; font-variant-numeric: inherit; font-variant-east-asian: inherit; font-variant-alternates: inherit; font-variant-position: inherit; font-variant-emoji: inherit; font-stretch: inherit; font-size: 12pt; line-height: inherit; font-family: Aptos, Aptos_EmbeddedFont, Aptos_MSFontService, Calibri, Helvetica, sans-serif; font-optical-sizing: inherit; font-size-adjust: inherit; font-kerning: inherit; font-feature-settings: inherit; font-variation-settings: inherit; margin: 1em 0px; padding: 0px; vertical-align: baseline;">구체적 HPLC 분석 조건(컬럼/용매/검출기) 및 논문별 분리 크로마토그램 사례<o:p></o:p>
>
>mso-bidi-font-family:"Segoe UI Emoji"">✅ 1️Polyamines (Spermidine, Spermine)<o:p></o:p>
>
>mso-bidi-font-family:"Segoe UI Emoji"">🔬 HPLC 분석
>조건 (대표)<o:p></o:p>
>
><table class="MsoNormalTable" border="0" cellpadding="0">
> <thead>
> <tr>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> 항목<o:p></o:p>
> </td>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> 조건<o:p></o:p>
> </td>
> </tr>
> </thead>
> <tbody><tr>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> 컬럼<o:p></o:p>
> </td>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> C18 역상 컬럼 (ex:
> Agilent ZORBAX Eclipse Plus C18, 4.6 × 150 mm, 5 μm)<o:p></o:p>
> </td>
> </tr>
> <tr>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> 이동상(Mobile Phase)<o:p></o:p>
> </td>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> A: 50 mM KH₂PO₄ (pH 6.8) + 5% ACN 
> B: ACN:MeOH:Water (45:45:10)<o:p></o:p>
> </td>
> </tr>
> <tr>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> 그라디언트<o:p></o:p>
> </td>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> 0–10 min: 10% B → 70% B (선형)<o:p></o:p>
> </td>
> </tr>
> <tr>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> 유속 (Flow rate)<o:p></o:p>
> </td>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> 1.0 mL/min<o:p></o:p>
> </td>
> </tr>
> <tr>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> 주입량<o:p></o:p>
> </td>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> 20 μL<o:p></o:p>
> </td>
> </tr>
> <tr>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> 검출기<o:p></o:p>
> </td>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> FLD (형광 검출, ex:
> Ex 340 nm / Em 445 nm)<o:p></o:p>
> </td>
> </tr>
> <tr>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> 파생화 시약<o:p></o:p>
> </td>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> OPA (Ortho-Phthalaldehyde), FMOC-Cl
> (Fluorenylmethyloxycarbonyl chloride)<o:p></o:p>
> </td>
> </tr>
> <tr>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> 전처리<o:p></o:p>
> </td>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> 시료 내 polyamine은
> OPA/FMOC로 유도체화 (15분 반응) 후
> 주입<o:p></o:p>
> </td>
> </tr>
></tbody></table>
>
>mso-bidi-font-family:"Segoe UI Emoji"">🔖 문헌
>예시<o:p></o:p>
>
><ul style="margin-top:0cm" type="disc">
> <li class="MsoNormal">Wu G. et al. Biology of
> Reproduction (2005). 
> → 돼지 태반 Proline → Putrescine/Spermidine 전환 경로
> 확인.<o:p></o:p></li>
></ul>
>
> 
>
>mso-bidi-font-family:"Segoe UI Emoji"">✅ 2️"Segoe UI Symbol"">⃣ Retinol (비타민 A)<o:p></o:p>
>
>mso-bidi-font-family:"Segoe UI Emoji"">🔬 HPLC 분석
>조건 (대표)<o:p></o:p>
>
><table class="MsoNormalTable" border="0" cellpadding="0">
> <thead>
> <tr>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> 항목<o:p></o:p>
> </td>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> 조건<o:p></o:p>
> </td>
> </tr>
> </thead>
> <tbody><tr>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> 컬럼<o:p></o:p>
> </td>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> C18 역상 컬럼 (Waters
> Symmetry C18, 4.6 × 250 mm, 5 μm)<o:p></o:p>
> </td>
> </tr>
> <tr>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> 이동상<o:p></o:p>
> </td>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> MeOH:Water (95:5, v/v) + 0.1% BHT (산화
> 방지)<o:p></o:p>
> </td>
> </tr>
> <tr>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> 유속<o:p></o:p>
> </td>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> 1.0 mL/min<o:p></o:p>
> </td>
> </tr>
> <tr>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> 주입량<o:p></o:p>
> </td>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> 20 μL<o:p></o:p>
> </td>
> </tr>
> <tr>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> 검출기<o:p></o:p>
> </td>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> UV 검출 (λ = 325
> nm)<o:p></o:p>
> </td>
> </tr>
> <tr>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> 전처리<o:p></o:p>
> </td>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> 헥산:EtOH 추출 → SPE
> (Silica) 정제 → 농축<o:p></o:p>
> </td>
> </tr>
></tbody></table>
>
>mso-bidi-font-family:"Segoe UI Emoji"">🔖 문헌
>예시<o:p></o:p>
>
><ul style="margin-top:0cm" type="disc">
> <li class="MsoNormal">Törmä H. et al. Placenta
> (1986). 
> → 태반 homogenate에서
> [³H]Retinol 분리 후 HPLC로 확인.<o:p></o:p></li>
> <li class="MsoNormal">SCCS 비타민 A 안전성 리포트에서 동일한 RP-HPLC 조건 활용됨.<o:p></o:p></li>
></ul>
>
> 
>
>mso-bidi-font-family:"Segoe UI Emoji"">✅ 3️"Segoe UI Symbol"">⃣ Arachidonic Acid (PUFA)<o:p></o:p>
>
>mso-bidi-font-family:"Segoe UI Emoji"">🔬 HPLC 분석
>조건 (대표)<o:p></o:p>
>
><table class="MsoNormalTable" border="0" cellpadding="0">
> <thead>
> <tr>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> 항목<o:p></o:p>
> </td>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> 조건<o:p></o:p>
> </td>
> </tr>
> </thead>
> <tbody><tr>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> 컬럼<o:p></o:p>
> </td>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> C18 역상 컬럼
> (Phenomenex Luna C18(2), 4.6 × 250 mm, 5 μm)<o:p></o:p>
> </td>
> </tr>
> <tr>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> 이동상<o:p></o:p>
> </td>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> A: Water + 0.1% H₃PO₄ 
> B: ACN:MeOH (85:15, v/v)<o:p></o:p>
> </td>
> </tr>
> <tr>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> 그라디언트<o:p></o:p>
> </td>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> 0–30 min: 50% B → 100% B<o:p></o:p>
> </td>
> </tr>
> <tr>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> 유속<o:p></o:p>
> </td>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> 1.0 mL/min<o:p></o:p>
> </td>
> </tr>
> <tr>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> 검출기<o:p></o:p>
> </td>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> UV 검출 (λ = 205
> nm)<o:p></o:p>
> </td>
> </tr>
> <tr>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> 파생화<o:p></o:p>
> </td>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> FAMEs 유도체화
> (BF₃-MeOH, 60℃, 30분)<o:p></o:p>
> </td>
> </tr>
> <tr>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> 전처리<o:p></o:p>
> </td>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> 헥산 추출 → FAMEs → 정제 후 주입<o:p></o:p>
> </td>
> </tr>
></tbody></table>
>
>mso-bidi-font-family:"Segoe UI Emoji"">🔖 문헌
>예시<o:p></o:p>
>
><ul style="margin-top:0cm" type="disc">
> <li class="MsoNormal">IGF-2 조절 지방산 논문 (Biol Reprod,
> 2005)<o:p></o:p></li>
> <li class="MsoNormal">표준 PUFA HPLC는 Lipids, J Chromatogr A 표준 조건 인용.<o:p></o:p></li>
></ul>
>
> 
>
>mso-bidi-font-family:"Segoe UI Emoji"">✅ 4️"Segoe UI Symbol"">⃣ 담즙산
>(Hyodeoxycholic acid 등)<o:p></o:p>
>
>mso-bidi-font-family:"Segoe UI Emoji"">🔬 HPLC 분석
>조건 (대표)<o:p></o:p>
>
><table class="MsoNormalTable" border="0" cellpadding="0">
> <thead>
> <tr>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> 항목<o:p></o:p>
> </td>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> 조건<o:p></o:p>
> </td>
> </tr>
> </thead>
> <tbody><tr>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> 컬럼<o:p></o:p>
> </td>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> C18 역상 컬럼 (Waters
> XBridge C18, 4.6 × 150 mm, 5 μm)<o:p></o:p>
> </td>
> </tr>
> <tr>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> 이동상<o:p></o:p>
> </td>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> A: 10 mM KH₂PO₄ (pH 2.5) 
> B: ACN<o:p></o:p>
> </td>
> </tr>
> <tr>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> 그라디언트<o:p></o:p>
> </td>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> 0–20 min: 30% B → 70% B<o:p></o:p>
> </td>
> </tr>
> <tr>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> 유속<o:p></o:p>
> </td>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> 1.0 mL/min<o:p></o:p>
> </td>
> </tr>
> <tr>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> 검출기<o:p></o:p>
> </td>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> UV 검출 (λ = 210
> nm) 또는 ELSD<o:p></o:p>
> </td>
> </tr>
> <tr>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> 전처리<o:p></o:p>
> </td>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> SPE 정제, 담즙산 Na-salt로 전환<o:p></o:p>
> </td>
> </tr>
> <tr>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> Ion-pairing<o:p></o:p>
> </td>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> Sodium heptanesulfonate (0.005 M) 가끔
> 첨가<o:p></o:p>
> </td>
> </tr>
></tbody></table>
>
>mso-bidi-font-family:"Segoe UI Emoji"">🔖 문헌
>예시<o:p></o:p>
>
><ul style="margin-top:0cm" type="disc">
> <li class="MsoNormal">Placental bile acid profiling, MDPI
> IJMS (2023). 
> → Human 태반 담즙산도 동일 방식 적용됨.<o:p></o:p></li>
></ul>
>
> 
>
>mso-bidi-font-family:"Segoe UI Emoji"">✅ 5️"Segoe UI Symbol"">⃣ 저분자 펩타이드<o:p></o:p>
>
>mso-bidi-font-family:"Segoe UI Emoji"">🔬 HPLC 분석
>조건 (대표)<o:p></o:p>
>
><table class="MsoNormalTable" border="0" cellpadding="0">
> <thead>
> <tr>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> 항목<o:p></o:p>
> </td>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> 조건<o:p></o:p>
> </td>
> </tr>
> </thead>
> <tbody><tr>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> 컬럼<o:p></o:p>
> </td>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> C18 역상 컬럼 (Vydac
> C18, 4.6 × 250 mm, 5 μm)<o:p></o:p>
> </td>
> </tr>
> <tr>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> 이동상<o:p></o:p>
> </td>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> A: 0.1% TFA in Water 
> B: 0.1% TFA in ACN<o:p></o:p>
> </td>
> </tr>
> <tr>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> 그라디언트<o:p></o:p>
> </td>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> 0–30 min: 5% B → 50% B<o:p></o:p>
> </td>
> </tr>
> <tr>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> 유속<o:p></o:p>
> </td>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> 1.0 mL/min<o:p></o:p>
> </td>
> </tr>
> <tr>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> 검출기<o:p></o:p>
> </td>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> UV 검출 (λ = 220
> nm)<o:p></o:p>
> </td>
> </tr>
> <tr>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> 전처리<o:p></o:p>
> </td>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> Alcalase 처리 → 10
> kDa 이하 ultrafiltration<o:p></o:p>
> </td>
> </tr>
> <tr>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> 분석 특징<o:p></o:p>
> </td>
> <td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
> padding:.75pt .75pt .75pt .75pt">
> 분획별 RT 기록 → LC–MS
> 연동 가능<o:p></o:p>
> </td>
> </tr>
></tbody></table>
>
>mso-bidi-font-family:"Segoe UI Emoji"">🔖 문헌
>예시<o:p></o:p>
>
><ul style="margin-top:0cm" type="disc">
> <li class="MsoNormal">Laosam et al. PLOS ONE
> (2021). 
> → 돼지 태반 가수분해물 펩타이드 HPLC 크로마토그램 수록.<o:p></o:p></li>
></ul>
>
> </div><div class="x_elementToProof" style="border: 0px; font-variant-numeric: inherit; font-variant-east-asian: inherit; font-variant-alternates: inherit; font-variant-position: inherit; font-variant-emoji: inherit; font-stretch: inherit; font-size: 12pt; line-height: inherit; font-family: Aptos, Aptos_EmbeddedFont, Aptos_MSFontService, Calibri, Helvetica, sans-serif; font-optical-sizing: inherit; font-size-adjust: inherit; font-kerning: inherit; font-feature-settings: inherit; font-variation-settings: inherit; margin: 1em 0px; padding: 0px; vertical-align: baseline;">감사합니다</div>

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