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운영자님의 글입니다. ><div>김민지님의 글의 답변 입니다.</div><div><br></div><div>문의주신 화장품과년 성분 분석은 우리원에서 하지 않습니다. </div><div>식품에 관한 식품공전에 등제된 시험을 하고 있습니다. </div><div><br></div><div>감사합니다. </div><div><br></div><div><br></div><div><br></div>김민지님의 글입니다. >>안녕하세요 <div><br></div><div>물리화학적 성질 분석 및 성분 분석 가능 여부 문의드립니다. 물리화학적 성분 아래 내용에서 어떤 것들이 평가 가능할까요? </div><div>돼지태반 추출물은 분말 상태로 되어있습니다.</div><div><br></div><div><div class="x_elementToProof" data-olk-copy-source="MessageBody" style="border: 0px; font-variant-numeric: inherit; font-variant-east-asian: inherit; font-variant-alternates: inherit; font-variant-position: inherit; font-variant-emoji: inherit; font-stretch: inherit; font-size: 12pt; line-height: inherit; font-family: Aptos, Aptos_EmbeddedFont, Aptos_MSFontService, Calibri, Helvetica, sans-serif; font-optical-sizing: inherit; font-size-adjust: inherit; font-kerning: inherit; font-feature-settings: inherit; font-variation-settings: inherit; margin: 0px; padding: 0px; vertical-align: baseline;">식약처 화장품 위해평가 보고서 작성을 위해 물리 화학적 성질 데이터가 필요합니다.</div><div class="x_elementToProof" style="border: 0px; font-variant-numeric: inherit; font-variant-east-asian: inherit; font-variant-alternates: inherit; font-variant-position: inherit; font-variant-emoji: inherit; font-stretch: inherit; font-size: 12pt; line-height: inherit; font-family: Aptos, Aptos_EmbeddedFont, Aptos_MSFontService, Calibri, Helvetica, sans-serif; font-optical-sizing: inherit; font-size-adjust: inherit; font-kerning: inherit; font-feature-settings: inherit; font-variation-settings: inherit; margin: 1em 0px; padding: 0px; vertical-align: baseline;">[물질명, IUPAC명, CAS No., 화학식, 분자량, 순도(분석방법), 구조식, 물리적 성상, 녹는점, 끓는점, 밀도, 용해도, Log Pow, 헨리상수, 동의어]</div></div><div class="x_elementToProof" style="border: 0px; font-variant-numeric: inherit; font-variant-east-asian: inherit; font-variant-alternates: inherit; font-variant-position: inherit; font-variant-emoji: inherit; font-stretch: inherit; font-size: 12pt; line-height: inherit; font-family: Aptos, Aptos_EmbeddedFont, Aptos_MSFontService, Calibri, Helvetica, sans-serif; font-optical-sizing: inherit; font-size-adjust: inherit; font-kerning: inherit; font-feature-settings: inherit; font-variation-settings: inherit; margin: 1em 0px; padding: 0px; vertical-align: baseline;">성분분석 의뢰 내용은 다음과 같습니다.</div><div class="x_elementToProof" style="border: 0px; font-variant-numeric: inherit; font-variant-east-asian: inherit; font-variant-alternates: inherit; font-variant-position: inherit; font-variant-emoji: inherit; font-stretch: inherit; font-size: 12pt; line-height: inherit; font-family: Aptos, Aptos_EmbeddedFont, Aptos_MSFontService, Calibri, Helvetica, sans-serif; font-optical-sizing: inherit; font-size-adjust: inherit; font-kerning: inherit; font-feature-settings: inherit; font-variation-settings: inherit; margin: 1em 0px; padding: 0px; vertical-align: baseline;">전처리도 해주시는지 궁금합니다.</div><div class="x_elementToProof" style="border: 0px; font-variant-numeric: inherit; font-variant-east-asian: inherit; font-variant-alternates: inherit; font-variant-position: inherit; font-variant-emoji: inherit; font-stretch: inherit; font-size: 12pt; line-height: inherit; font-family: Aptos, Aptos_EmbeddedFont, Aptos_MSFontService, Calibri, Helvetica, sans-serif; font-optical-sizing: inherit; font-size-adjust: inherit; font-kerning: inherit; font-feature-settings: inherit; font-variation-settings: inherit; margin: 1em 0px; padding: 0px; vertical-align: baseline;"><p class="MsoNormal"><b>구체적<span lang="EN-US"> HPLC </span>분석 조건<span lang="EN-US">(</span>컬럼<span lang="EN-US">/</span>용매<span lang="EN-US">/</span>검출기<span lang="EN-US">)</span></b><span lang="EN-US"> </span>및 <b>논문별 분리 크로마토그램 사례<span lang="EN-US"><o:p></o:p></span></b></p> >> >><p class="MsoNormal"><b><span lang="EN-US" style="font-family:" segoe="" ui="" emoji",sans-serif;="">mso-bidi-font-family:"Segoe UI Emoji"">✅</span><span lang="EN-US"> 1️</span></b><b><span lang="EN-US">Polyamines (Spermidine, Spermine)<o:p></o:p></span></b></p> >> >><p class="MsoNormal"><b><span lang="EN-US" style="font-family:" segoe="" ui="" emoji",sans-serif;="">mso-bidi-font-family:"Segoe UI Emoji"">🔬</span><span lang="EN-US"> HPLC </span>분석 >>조건<span lang="EN-US"> (</span>대표<span lang="EN-US">)<o:p></o:p></span></b></p> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >><table class="MsoNormalTable" border="0" cellpadding="0"><thead><tr><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><b>항목<span lang="EN-US"><o:p></o:p></span></b></p> >> </td><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><b>조건<span lang="EN-US"><o:p></o:p></span></b></p> >> </td></tr></thead><tbody><tr><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><b>컬럼</b><span lang="EN-US"><o:p></o:p></span></p> >> </td><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><span lang="EN-US">C18 </span>역상 컬럼<span lang="EN-US"> (ex: >> Agilent ZORBAX Eclipse Plus C18, 4.6 × 150 mm, 5 μm)<o:p></o:p></span></p> >> </td></tr><tr><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><b>이동상<span lang="EN-US">(Mobile Phase)</span></b><span lang="EN-US"><o:p></o:p></span></p> >> </td><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><span lang="EN-US">A: 50 mM KH₂PO₄ (pH 6.8) + 5% ACN<br> >> B: ACN:MeOH:Water (45:45:10)<o:p></o:p></span></p> >> </td></tr><tr><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><b>그라디언트</b><span lang="EN-US"><o:p></o:p></span></p> >> </td><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><span lang="EN-US">0–10 min: 10% B → 70% B (</span>선형<span lang="EN-US">)<o:p></o:p></span></p> >> </td></tr><tr><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><b>유속<span lang="EN-US"> (Flow rate)</span></b><span lang="EN-US"><o:p></o:p></span></p> >> </td><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><span lang="EN-US">1.0 mL/min<o:p></o:p></span></p> >> </td></tr><tr><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><b>주입량</b><span lang="EN-US"><o:p></o:p></span></p> >> </td><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><span lang="EN-US">20 μL<o:p></o:p></span></p> >> </td></tr><tr><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><b>검출기</b><span lang="EN-US"><o:p></o:p></span></p> >> </td><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><span lang="EN-US">FLD (</span>형광 검출<span lang="EN-US">, ex: >> Ex 340 nm / Em 445 nm)<o:p></o:p></span></p> >> </td></tr><tr><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><b>파생화 시약</b><span lang="EN-US"><o:p></o:p></span></p> >> </td><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><span lang="EN-US">OPA (Ortho-Phthalaldehyde), FMOC-Cl >> (Fluorenylmethyloxycarbonyl chloride)<o:p></o:p></span></p> >> </td></tr><tr><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><b>전처리</b><span lang="EN-US"><o:p></o:p></span></p> >> </td><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal">시료 내<span lang="EN-US"> polyamine</span>은<span lang="EN-US"> >> OPA/FMOC</span>로 유도체화<span lang="EN-US"> (15</span>분 반응<span lang="EN-US">) </span>후 >> 주입<span lang="EN-US"><o:p></o:p></span></p> >> </td></tr></tbody></table> >> >><p class="MsoNormal"><b><span lang="EN-US" style="font-family:" segoe="" ui="" emoji",sans-serif;="">mso-bidi-font-family:"Segoe UI Emoji"">🔖</span><span lang="EN-US"> </span>문헌 >>예시<span lang="EN-US"><o:p></o:p></span></b></p> >> >><ul style="margin-top:0cm" type="disc"> >> <li class="MsoNormal"><b><span lang="EN-US">Wu G. et al.</span></b><span lang="EN-US"> <i>Biology of >> Reproduction</i> (2005).<br> >> → </span>돼지 태반<span lang="EN-US"> Proline → Putrescine/Spermidine </span>전환 경로 >> 확인<span lang="EN-US">.<o:p></o:p></span></li> >></ul> >> >><p class="MsoNormal"><span lang="EN-US"> </span></p> >> >><p class="MsoNormal"><b><span lang="EN-US" style="font-family:" segoe="" ui="" emoji",sans-serif;="">mso-bidi-font-family:"Segoe UI Emoji"">✅</span><span lang="EN-US"> 2️</span></b><b><span lang="EN-US" style="font-family:" segoe="" ui="" symbol",sans-serif;mso-bidi-font-family:="">"Segoe UI Symbol"">⃣</span><span lang="EN-US"> Retinol (</span>비타민<span lang="EN-US"> A)<o:p></o:p></span></b></p> >> >><p class="MsoNormal"><b><span lang="EN-US" style="font-family:" segoe="" ui="" emoji",sans-serif;="">mso-bidi-font-family:"Segoe UI Emoji"">🔬</span><span lang="EN-US"> HPLC </span>분석 >>조건<span lang="EN-US"> (</span>대표<span lang="EN-US">)<o:p></o:p></span></b></p> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >><table class="MsoNormalTable" border="0" cellpadding="0"><thead><tr><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><b>항목<span lang="EN-US"><o:p></o:p></span></b></p> >> </td><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><b>조건<span lang="EN-US"><o:p></o:p></span></b></p> >> </td></tr></thead><tbody><tr><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><b>컬럼</b><span lang="EN-US"><o:p></o:p></span></p> >> </td><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><span lang="EN-US">C18 </span>역상 컬럼<span lang="EN-US"> (Waters >> Symmetry C18, 4.6 × 250 mm, 5 μm)<o:p></o:p></span></p> >> </td></tr><tr><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><b>이동상</b><span lang="EN-US"><o:p></o:p></span></p> >> </td><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><span lang="EN-US">MeOH:Water (95:5, v/v) + 0.1% BHT (</span>산화 >> 방지<span lang="EN-US">)<o:p></o:p></span></p> >> </td></tr><tr><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><b>유속</b><span lang="EN-US"><o:p></o:p></span></p> >> </td><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><span lang="EN-US">1.0 mL/min<o:p></o:p></span></p> >> </td></tr><tr><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><b>주입량</b><span lang="EN-US"><o:p></o:p></span></p> >> </td><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><span lang="EN-US">20 μL<o:p></o:p></span></p> >> </td></tr><tr><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><b>검출기</b><span lang="EN-US"><o:p></o:p></span></p> >> </td><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><span lang="EN-US">UV </span>검출<span lang="EN-US"> (λ = 325 >> nm)<o:p></o:p></span></p> >> </td></tr><tr><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><b>전처리</b><span lang="EN-US"><o:p></o:p></span></p> >> </td><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal">헥산<span lang="EN-US">:EtOH </span>추출<span lang="EN-US"> → SPE >> (Silica) </span>정제<span lang="EN-US"> → </span>농축<span lang="EN-US"><o:p></o:p></span></p> >> </td></tr></tbody></table> >> >><p class="MsoNormal"><b><span lang="EN-US" style="font-family:" segoe="" ui="" emoji",sans-serif;="">mso-bidi-font-family:"Segoe UI Emoji"">🔖</span><span lang="EN-US"> </span>문헌 >>예시<span lang="EN-US"><o:p></o:p></span></b></p> >> >><ul style="margin-top:0cm" type="disc"> >> <li class="MsoNormal"><b><span lang="EN-US">Törmä H. et al.</span></b><span lang="EN-US"> <i>Placenta</i> >> (1986).<br> >> → </span>태반<span lang="EN-US"> homogenate</span>에서<span lang="EN-US"> >> [³H]Retinol </span>분리 후<span lang="EN-US"> HPLC</span>로 확인<span lang="EN-US">.<o:p></o:p></span></li> >> <li class="MsoNormal"><span lang="EN-US">SCCS </span>비타민<span lang="EN-US"> A </span>안전성 리포트에서 동일한<span lang="EN-US"> RP-HPLC </span>조건 활용됨<span lang="EN-US">.<o:p></o:p></span></li> >></ul> >> >><p class="MsoNormal"><span lang="EN-US"> </span></p> >> >><p class="MsoNormal"><b><span lang="EN-US" style="font-family:" segoe="" ui="" emoji",sans-serif;="">mso-bidi-font-family:"Segoe UI Emoji"">✅</span><span lang="EN-US"> 3️</span></b><b><span lang="EN-US" style="font-family:" segoe="" ui="" symbol",sans-serif;mso-bidi-font-family:="">"Segoe UI Symbol"">⃣</span><span lang="EN-US"> Arachidonic Acid (PUFA)<o:p></o:p></span></b></p> >> >><p class="MsoNormal"><b><span lang="EN-US" style="font-family:" segoe="" ui="" emoji",sans-serif;="">mso-bidi-font-family:"Segoe UI Emoji"">🔬</span><span lang="EN-US"> HPLC </span>분석 >>조건<span lang="EN-US"> (</span>대표<span lang="EN-US">)<o:p></o:p></span></b></p> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >><table class="MsoNormalTable" border="0" cellpadding="0"><thead><tr><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><b>항목<span lang="EN-US"><o:p></o:p></span></b></p> >> </td><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><b>조건<span lang="EN-US"><o:p></o:p></span></b></p> >> </td></tr></thead><tbody><tr><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><b>컬럼</b><span lang="EN-US"><o:p></o:p></span></p> >> </td><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><span lang="EN-US">C18 </span>역상 컬럼<span lang="EN-US"> >> (Phenomenex Luna C18(2), 4.6 × 250 mm, 5 μm)<o:p></o:p></span></p> >> </td></tr><tr><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><b>이동상</b><span lang="EN-US"><o:p></o:p></span></p> >> </td><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><span lang="EN-US">A: Water + 0.1% H₃PO₄<br> >> B: ACN:MeOH (85:15, v/v)<o:p></o:p></span></p> >> </td></tr><tr><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><b>그라디언트</b><span lang="EN-US"><o:p></o:p></span></p> >> </td><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><span lang="EN-US">0–30 min: 50% B → 100% B<o:p></o:p></span></p> >> </td></tr><tr><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><b>유속</b><span lang="EN-US"><o:p></o:p></span></p> >> </td><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><span lang="EN-US">1.0 mL/min<o:p></o:p></span></p> >> </td></tr><tr><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><b>검출기</b><span lang="EN-US"><o:p></o:p></span></p> >> </td><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><span lang="EN-US">UV </span>검출<span lang="EN-US"> (λ = 205 >> nm)<o:p></o:p></span></p> >> </td></tr><tr><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><b>파생화</b><span lang="EN-US"><o:p></o:p></span></p> >> </td><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><span lang="EN-US">FAMEs </span>유도체화<span lang="EN-US"> >> (BF₃-MeOH, 60℃, 30</span>분<span lang="EN-US">)<o:p></o:p></span></p> >> </td></tr><tr><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><b>전처리</b><span lang="EN-US"><o:p></o:p></span></p> >> </td><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal">헥산 추출<span lang="EN-US"> → FAMEs → </span>정제 후 주입<span lang="EN-US"><o:p></o:p></span></p> >> </td></tr></tbody></table> >> >><p class="MsoNormal"><b><span lang="EN-US" style="font-family:" segoe="" ui="" emoji",sans-serif;="">mso-bidi-font-family:"Segoe UI Emoji"">🔖</span><span lang="EN-US"> </span>문헌 >>예시<span lang="EN-US"><o:p></o:p></span></b></p> >> >><ul style="margin-top:0cm" type="disc"> >> <li class="MsoNormal"><span lang="EN-US">IGF-2 </span>조절 지방산 논문<span lang="EN-US"> (<i>Biol Reprod</i>, >> 2005)<o:p></o:p></span></li> >> <li class="MsoNormal">표준<span lang="EN-US"> PUFA HPLC</span>는 <i><span lang="EN-US">Lipids</span></i><span lang="EN-US">, <i>J Chromatogr A</i> </span>표준 조건 인용<span lang="EN-US">.<o:p></o:p></span></li> >></ul> >> >><p class="MsoNormal"><span lang="EN-US"> </span></p> >> >><p class="MsoNormal"><b><span lang="EN-US" style="font-family:" segoe="" ui="" emoji",sans-serif;="">mso-bidi-font-family:"Segoe UI Emoji"">✅</span><span lang="EN-US"> 4️</span></b><b><span lang="EN-US" style="font-family:" segoe="" ui="" symbol",sans-serif;mso-bidi-font-family:="">"Segoe UI Symbol"">⃣</span><span lang="EN-US"> </span>담즙산<span lang="EN-US"> >>(Hyodeoxycholic acid </span>등<span lang="EN-US">)<o:p></o:p></span></b></p> >> >><p class="MsoNormal"><b><span lang="EN-US" style="font-family:" segoe="" ui="" emoji",sans-serif;="">mso-bidi-font-family:"Segoe UI Emoji"">🔬</span><span lang="EN-US"> HPLC </span>분석 >>조건<span lang="EN-US"> (</span>대표<span lang="EN-US">)<o:p></o:p></span></b></p> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >><table class="MsoNormalTable" border="0" cellpadding="0"><thead><tr><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><b>항목<span lang="EN-US"><o:p></o:p></span></b></p> >> </td><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><b>조건<span lang="EN-US"><o:p></o:p></span></b></p> >> </td></tr></thead><tbody><tr><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><b>컬럼</b><span lang="EN-US"><o:p></o:p></span></p> >> </td><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><span lang="EN-US">C18 </span>역상 컬럼<span lang="EN-US"> (Waters >> XBridge C18, 4.6 × 150 mm, 5 μm)<o:p></o:p></span></p> >> </td></tr><tr><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><b>이동상</b><span lang="EN-US"><o:p></o:p></span></p> >> </td><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><span lang="EN-US">A: 10 mM KH₂PO₄ (pH 2.5)<br> >> B: ACN<o:p></o:p></span></p> >> </td></tr><tr><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><b>그라디언트</b><span lang="EN-US"><o:p></o:p></span></p> >> </td><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><span lang="EN-US">0–20 min: 30% B → 70% B<o:p></o:p></span></p> >> </td></tr><tr><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><b>유속</b><span lang="EN-US"><o:p></o:p></span></p> >> </td><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><span lang="EN-US">1.0 mL/min<o:p></o:p></span></p> >> </td></tr><tr><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><b>검출기</b><span lang="EN-US"><o:p></o:p></span></p> >> </td><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><span lang="EN-US">UV </span>검출<span lang="EN-US"> (λ = 210 >> nm) </span>또는<span lang="EN-US"> ELSD<o:p></o:p></span></p> >> </td></tr><tr><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><b>전처리</b><span lang="EN-US"><o:p></o:p></span></p> >> </td><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><span lang="EN-US">SPE </span>정제<span lang="EN-US">, </span>담즙산<span lang="EN-US"> Na-salt</span>로 전환<span lang="EN-US"><o:p></o:p></span></p> >> </td></tr><tr><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><b><span lang="EN-US">Ion-pairing</span></b><span lang="EN-US"><o:p></o:p></span></p> >> </td><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><span lang="EN-US">Sodium heptanesulfonate (0.005 M) </span>가끔 >> 첨가<span lang="EN-US"><o:p></o:p></span></p> >> </td></tr></tbody></table> >> >><p class="MsoNormal"><b><span lang="EN-US" style="font-family:" segoe="" ui="" emoji",sans-serif;="">mso-bidi-font-family:"Segoe UI Emoji"">🔖</span><span lang="EN-US"> </span>문헌 >>예시<span lang="EN-US"><o:p></o:p></span></b></p> >> >><ul style="margin-top:0cm" type="disc"> >> <li class="MsoNormal"><b><span lang="EN-US">Placental bile acid profiling</span></b><span lang="EN-US">, <i>MDPI >> IJMS</i> (2023).<br> >> → Human </span>태반 담즙산도 동일 방식 적용됨<span lang="EN-US">.<o:p></o:p></span></li> >></ul> >> >><p class="MsoNormal"><span lang="EN-US"> </span></p> >> >><p class="MsoNormal"><b><span lang="EN-US" style="font-family:" segoe="" ui="" emoji",sans-serif;="">mso-bidi-font-family:"Segoe UI Emoji"">✅</span><span lang="EN-US"> 5️</span></b><b><span lang="EN-US" style="font-family:" segoe="" ui="" symbol",sans-serif;mso-bidi-font-family:="">"Segoe UI Symbol"">⃣</span><span lang="EN-US"> </span>저분자 펩타이드<span lang="EN-US"><o:p></o:p></span></b></p> >> >><p class="MsoNormal"><b><span lang="EN-US" style="font-family:" segoe="" ui="" emoji",sans-serif;="">mso-bidi-font-family:"Segoe UI Emoji"">🔬</span><span lang="EN-US"> HPLC </span>분석 >>조건<span lang="EN-US"> (</span>대표<span lang="EN-US">)<o:p></o:p></span></b></p> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >><table class="MsoNormalTable" border="0" cellpadding="0"><thead><tr><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><b>항목<span lang="EN-US"><o:p></o:p></span></b></p> >> </td><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><b>조건<span lang="EN-US"><o:p></o:p></span></b></p> >> </td></tr></thead><tbody><tr><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><b>컬럼</b><span lang="EN-US"><o:p></o:p></span></p> >> </td><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><span lang="EN-US">C18 </span>역상 컬럼<span lang="EN-US"> (Vydac >> C18, 4.6 × 250 mm, 5 μm)<o:p></o:p></span></p> >> </td></tr><tr><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><b>이동상</b><span lang="EN-US"><o:p></o:p></span></p> >> </td><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><span lang="EN-US">A: 0.1% TFA in Water<br> >> B: 0.1% TFA in ACN<o:p></o:p></span></p> >> </td></tr><tr><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><b>그라디언트</b><span lang="EN-US"><o:p></o:p></span></p> >> </td><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><span lang="EN-US">0–30 min: 5% B → 50% B<o:p></o:p></span></p> >> </td></tr><tr><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><b>유속</b><span lang="EN-US"><o:p></o:p></span></p> >> </td><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><span lang="EN-US">1.0 mL/min<o:p></o:p></span></p> >> </td></tr><tr><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><b>검출기</b><span lang="EN-US"><o:p></o:p></span></p> >> </td><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><span lang="EN-US">UV </span>검출<span lang="EN-US"> (λ = 220 >> nm)<o:p></o:p></span></p> >> </td></tr><tr><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><b>전처리</b><span lang="EN-US"><o:p></o:p></span></p> >> </td><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><span lang="EN-US">Alcalase </span>처리<span lang="EN-US"> → 10 >> kDa </span>이하<span lang="EN-US"> ultrafiltration<o:p></o:p></span></p> >> </td></tr><tr><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal"><b>분석 특징</b><span lang="EN-US"><o:p></o:p></span></p> >> </td><td style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt; >> padding:.75pt .75pt .75pt .75pt"> >> <p class="MsoNormal">분획별<span lang="EN-US"> RT </span>기록<span lang="EN-US"> → LC–MS >> </span>연동 가능<span lang="EN-US"><o:p></o:p></span></p> >> </td></tr></tbody></table> >> >><p class="MsoNormal"><b><span lang="EN-US" style="font-family:" segoe="" ui="" emoji",sans-serif;="">mso-bidi-font-family:"Segoe UI Emoji"">🔖</span><span lang="EN-US"> </span>문헌 >>예시<span lang="EN-US"><o:p></o:p></span></b></p> >> >><ul style="margin-top:0cm" type="disc"> >> <li class="MsoNormal"><b><span lang="EN-US">Laosam et al.</span></b><span lang="EN-US"> <i>PLOS ONE</i> >> (2021).<br> >> → </span>돼지 태반 가수분해물 펩타이드<span lang="EN-US"> HPLC </span>크로마토그램 수록<span lang="EN-US">.<o:p></o:p></span></li> >></ul> >> >><p class="MsoNormal"><span lang="EN-US"> </span></p></div><div class="x_elementToProof" style="border: 0px; font-variant-numeric: inherit; font-variant-east-asian: inherit; font-variant-alternates: inherit; font-variant-position: inherit; font-variant-emoji: inherit; font-stretch: inherit; font-size: 12pt; line-height: inherit; font-family: Aptos, Aptos_EmbeddedFont, Aptos_MSFontService, Calibri, Helvetica, sans-serif; font-optical-sizing: inherit; font-size-adjust: inherit; font-kerning: inherit; font-feature-settings: inherit; font-variation-settings: inherit; margin: 1em 0px; padding: 0px; vertical-align: baseline;">감사합니다</div>
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